ABSTRACT
OBJECTIVE: To investigate the effect of vitamin A supplementation on the retinol concentration in colostrum under fasting and postprandial conditions. METHODS: This was a quasi-experimental study, with before and after assessments, conducted with 33 patients treated at a public maternity hospital. Blood and colostrum samples were collected under fasting conditions in the immediate postpartum period. A second colostrum collection occurred two hours after the first meal of the day, at which time a mega dose of 200,000 IU of retinyl palmitate was administered. On the following day, the colostrum was collected again under fasting and postprandial conditions. Serum and colostrum retinol concentrations were determined by high performance liquid chromatography. RESULTS: The serum retinol concentration was 37.3 (16.8-62.2) µg/dL, indicating adequate nutritional status. The colostrum retinol concentration before supplementation was 46.8 (29.7-158.9) µg/dL in fasting and 67.3 (31.1-148.7) µg/dL in postprandial condition (p < 0.05), showing an increase of 43.8%. After supplementation, the values were 89.5 (32.9-264.2) µg/dL and 102.7 (37.3-378.3) µg/dL in fasting and postprandial conditions, respectively (p < 0.05), representing an increase of 14.7%. CONCLUSIONS: This study demonstrated that maternal supplementation with high doses of vitamin A in postpartum resulted in a significant increase of the retinol concentration in colostrum under fasting conditions, with an even greater increase after a meal. .
OBJETIVO: Investigar o efeito da suplementação com vitamina A sobre a concentração de retinol no leite colostro em condições de jejum e pós-prandial. MÉTODOS: Estudo quase-experimental, do tipo antes e depois, realizado com 33 parturientes atendidas em uma maternidade pública, das quais foram coletadas, em jejum, amostras de sangue e leite colostro, no pós-parto imediato. Uma segunda coleta de colostro ocorreu duas horas após a primeira refeição do dia, momento em que uma megadose de 200.000 UI de palmitato de retinila foi administrada. No dia seguinte, uma nova coleta de colostro foi realizada em condições de jejum e pós-prandial. As concentrações de retinol no soro e no colostro foram determinadas por cromatografia líquida de alta eficiência. RESULTADOS: A concentração de retinol sérico foi de 37,3 (16,8-62,2) µg/dL, evidenciando um estado nutricional adequado. No colostro, a concentração de retinol antes da suplementação foi de 46,8 (29,7-158,9) µg/dL em jejum e 67,3 (31,1-148,7) µg/dL em condições pós-prandiais (p < 0,05), mostrando um aumento de 43,8%. Após a suplementação, os valores foram de 89,5 (32,9-264,2) µg/dL e 102,7 (37,3-378,3) µg/dL em jejum e pós-prandial, respectivamente (p < 0,05), representando um aumento de 14,7%. CONCLUSÕES: Este trabalho demonstrou que a suplementação materna com altas doses de vitamina A no pós-parto resultou em um aumento significativo da concentração de retinol no colostro em condições de jejum, sendo este valor ainda maior após a refeição. .
Subject(s)
Adolescent , Adult , Female , Humans , Pregnancy , Young Adult , Colostrum/chemistry , Dietary Supplements , Vitamin A/analysis , Vitamin A/analogs & derivatives , Chromatography, High Pressure Liquid , Colostrum/drug effects , Fasting/metabolism , Non-Randomized Controlled Trials as Topic , Postpartum Period , Postprandial Period , Vitamin A/administration & dosage , Vitamin A/blood , Vitamin A/pharmacokineticsABSTRACT
The aim of this study is to evaluate serum vitamin A levels in patients with rhinitis. A case control study concerned with 48 patients with rhinitis who underwent serum vitamin A evaluation and compared with the corresponding results of 100 apparently healthy persons as a control group. The study was carried out in Department of ENT in Al-Salam Teaching Hospital in Mosul, from April 2001 to June 2004. Vitamin A evaluation was performed in Department of Biochemistry in Mosul Medical College. The parameters analyzed included age, sex, clinical diagnosis and vitamin A measurement. The average age was 25 years with a range of 3-52 years. It was found that patients with rhinitis had significantly lower serum vitamin A levels than the control subjects. The levels were 0.292 +/- 0.099 [SD] micro mol/liter versus 0.886 +/- 0.199 [SD] micro mol/ liter, respectively. These findings prompted us to suggest a hypothesis that in rhinitis and possibly in other respiratory tract infections, a general derangement in vitamin A handling exists
Subject(s)
Humans , Male , Female , Vitamin A/pharmacokinetics , Vitamin A Deficiency/complications , Case-Control Studies , Rhinitis/etiology , Rhinitis/metabolism , Respiratory Tract Infections/etiology , Respiratory Tract Infections/metabolismABSTRACT
The purpose of this study was to evaluate the change in concentration of retinol and beta-carotene (BC) in blood serum and liver tissue of rats, after supplementation with synthetic BC and commonly consumed carotenoid-rich vegetables (carrot and spinach). Weanling male Wistar rats were randomly assigned in four groups of 16 rats each. The four groups of rats were supplemented according to the following feeding treatments: 1) Control group (0G), 0.2 mL corn oil; 2) Pure BC group (BCG), 60 micrograms RE in 0.2 mL corn oil; 3) Carrot group (CG), 60 micrograms RE in 0.2 mL corn oil; 4) Spinach group (SG), 60 micrograms RE in 0.2 mL corn oil. Analysis of retinol and BC contents in serum and liver was performed by HPLC procedures. The variance analysis showed no significant differences (a = 0.05) in the increase of weight of the animals and in the increase of retinol and BC levels in serum and in liver of the four treatments during the four weeks of supplementation. The correlation analysis between levels of retinol and BC in serum and in liver showed no relation between these two parameters. A regression analysis of liver BC levels in the four treatments showed the following slopes of the regression lines: BCG, 0.909; CG, 0.451; SG, 0.444, and 0G, 0.203. These results indicate that the highest BC absorption was in the BCG treatment, whereas the BC absorption in the CG and SG treatments was approximately one half.
Subject(s)
Animals , Male , Antioxidants/pharmacokinetics , Dietary Supplements , Liver/metabolism , beta Carotene/pharmacokinetics , Antioxidants/analysis , Biological Availability , Daucus carota , Liver/chemistry , Rats, Wistar , Spinacia oleracea , Vitamin A/analysis , Vitamin A/pharmacokinetics , beta Carotene/analysisABSTRACT
La vitamina A y sus derivados conocidos como retinoides (de origen animal) y compuestos pro-vitamina A denominados carotenoides (de origen vegetal) son importantes en la prevención de cáncer, enfermedades crónicas y enfermedades relacionadas con la deficiencia de vitamina A; por tanto, es importante conocer la absorción, metabolismo transporte y almacenamiento de estos compuestos en humanos. Debido a lo complejo que ha sido la utilización de modelos humanos para estudiar la biodisponibilidad de carotenoides de fuentes naturales y sintéticas, recientemente se han desarrollado modelos aniomales que permiten avances significativos en áreas de poco conocimiento. Esta revisión pretende dar la mayor información acerca de la farmacocinética y el metabolismo de este nutriente que permita a los interesados utilizar el modelo más apropiado para los fines que persiga